Mouse 썸네일형 리스트형 Mouse stereotaxic surgery Protocol Mouse stereotaxic surgery for injection 1. Measure weight of mice with balance to decide amount of anesthetics. 2. Prepare Ketamine/Xylazine in 1 cc syringe (28G ½) based on mice’s weight. a. Ketamine / Xylazine Mixture (Ketamine - 10 mg/ml, Xylazine - 1 mg/ml) - Ketamine - 100 mg/Kg dose, 10 mg/Kg dose 3. Put mice in isoflurane for about 5-10 seconds and take out 4. IP injection of Ket.. 더보기 Mouse genotyping HOLD THE AGAROSE! ADVANCED PCR METHODS FOR GENOTYPING MICE The intention of this article is to serve as a genotyping “primer” to familiarize you with the newer techniques that JAX uses to genotype mice in our repository, and to decode some of the sometimes puzzling terminology in our updated genotyping protocols. The JAX Technical Information Services (TIS) group answers between 130 and 200 ques.. 더보기 이전 1 다음
