Protocol 썸네일형 리스트형 Cell Subculture protocol Cell Subculture protocol. Junchul 1. Remove and discard the spent cell culture media from the culture dish. 2. Wash cells using a SF media (approximately 2 mL per 10 cm2 culture surface area). Gently add wash solution to the side of the dish opposite the attached cell layer to avoid disturbing the cell layer, and rock the vessel back and forth several times. 3. Remove and discard the wash soluti.. 더보기 Mouse stereotaxic surgery Protocol Mouse stereotaxic surgery for injection 1. Measure weight of mice with balance to decide amount of anesthetics. 2. Prepare Ketamine/Xylazine in 1 cc syringe (28G ½) based on mice’s weight. a. Ketamine / Xylazine Mixture (Ketamine - 10 mg/ml, Xylazine - 1 mg/ml) - Ketamine - 100 mg/Kg dose, 10 mg/Kg dose 3. Put mice in isoflurane for about 5-10 seconds and take out 4. IP injection of Ket.. 더보기 이전 1 다음
